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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in lots of tumors, which ends up in chromosome instability, and so could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial role in endorsing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two more splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however readily available.
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Blocking peptides are peptides that bind precisely for the goal antibody and block antibody binding. These peptide commonly is made up of the epitope acknowledged from the antibody.
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This guide outlines additional attainable optimization of mobile and immunoassay parameters to make sure the best possible final results are...
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This information outlines further more possible optimization of cellular and immunoassay parameters to make certain the very best effects are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.
Goal Info Cyclin E belongs towards the extremely conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary which is degraded as cells development by S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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